yoaE [2018-01-03 17:35:29]

formate dehydrogenase

locus: BSU18570

pI: 7.33

mw: 76.06 kDa

protein length: 677 aa

gene length: 2031 bp

function: unknown

product: formate dehydrogenase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]

Gene

Coordinates: 2,025,400 → 2,027,442

The protein

Paralogous protein(s)

• [protein|FEE1952DFA3D6A5E2DC108B3B203B526642E2C68|YyaE]

[SW|Cofactors]

• contains an iron-sulfur cluster

Structure

• [PDB|2VPW] (from Thermus thermophilus, 25% identity) [pubmed|18536726]

Expression and Regulation

Operons

• genes: [gene|628CCADB3C9A5F8A128C44ADE1DFDC2FC6B3CD01|yoaE]-[gene|78B66D5B9848D8247D8B9A0DE8923F50F8FCDB46|yoaF]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A836 (yoaE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/836 NBRP B. subtilis, Japan]
• BKE18570 (Δ[gene|628CCADB3C9A5F8A128C44ADE1DFDC2FC6B3CD01|yoaE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCCCAGTTCCTCTCTC, downstream forward: _UP4_TGAAAAATAATGAAACCCGG
• BKK18570 (Δ[gene|628CCADB3C9A5F8A128C44ADE1DFDC2FC6B3CD01|yoaE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCCCAGTTCCTCTCTC, downstream forward: _UP4_TGAAAAATAATGAAACCCGG

References

Research papers

• 18536726