SubtiBank SubtiBank
rsbR
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rsbR

[SW|stressosome] sensor protein, control of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] activity
locus
BSU_04670
pI
4.73
mw
30.90 kDa
protein length
274 aa Sequence Blast
gene length
825 bp Sequence Blast
function
control of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] activity
product
activator of [protein|search|RsbT ]kinase activity, [SW|stressosome] sensor protein
essential
no
synonyms
ycxR,rsbRA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • Gene

    Coordinates
    519,408 520,232

    The protein

    Catalyzed reaction/ biological activity

  • positive regulator of [protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA]-dependent light activation of the [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB] stress response [Pubmed|22287516]
  • phosphorylated [protein|621357D1FB6AC1B0499732EFE226F982B26CE34E|RsbR] activates the kinase activity of [protein|ADC52A22950736A0435AEEEC43F7407878786A81|RsbT] [Pubmed|23320651]
  • Paralogous protein(s)

  • [protein|6C5397BA5839E4DB25B370B39B6DA702147DA8B7|YtvA], [protein|979D7A45EAD97C99015029400A85795061BAA367|RsbRD], [protein|F53C527995909A1CFA72C7BF919DD10EE175C6D7|RsbRB], [protein|5EEEDE60CE9E5CDAEBAB0E03AABDF1DF62F1F002|RsbRC]
  • [SW|Domains]

  • RsbRA is composed of an N-terminal nonheme globin domain and a highly conserved C-terminal STAS (Sulphate Transporter and AntiSigma factor antagonist) domain. The C-terminal STAS domain is the target of the serine/threonine-specific kinase [protein|ADC52A22950736A0435AEEEC43F7407878786A81|RsbT] (see below).
  • Modification

  • phosphorylation on Thr-171 and Thr-205 by [protein|ADC52A22950736A0435AEEEC43F7407878786A81|RsbT] [Pubmed|21362065]
  • Structure

  • [PDB|2BNL] (N-terminal domain), [PDB|3VY9] (complete stressosome)
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR]-[gene|841CE7FCA4E84445830CA18F9856F6F30014E3BB|rsbS]-[gene|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT]-[gene|0B8381D2C724DAA724E4AFC67000F12AD112D13D|rsbU]-[gene|AC64DA463250A090A62E50901EFE653C8F963872|rsbV]-[gene|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|rsbW]-[gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]
    description
    [Pubmed|8002610]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8002610], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|20454630], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • regulation

  • ''[protein|search|rsbV]:'' induced by stress ([protein|search|SigB]) [Pubmed|11544224]
  • view in new tab

    Biological materials

    Mutant

  • BKE04670 ([gene|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATTCGCTTACCTCCCAA, downstream forward: _UP4_ATCGTTTCATTGGGGGAATA
  • BKK04670 ([gene|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATTCGCTTACCTCCCAA, downstream forward: _UP4_ATCGTTTCATTGGGGGAATA
  • labs

  • [SW|Bill Haldenwang], San Antonio, USA
  • [SW|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]
  • [SW|Rick Lewis], Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/r.lewis homepage]
  • References

    Reviews

  • 19704888,16319496,20658979,23416074,28271471
  • Original publications

  • 8002610,8682769,8682789,17726680,10781545,15583165,8824586,10329124,17158665,9179850,8808936,15312768,11244072,15342582,15378759,12950928,15466036,9179850,8955331,18832644,17726680,17218307,20019076,21602359,23320651,21362065,20935101,22287516,22609918,24599254,28727759