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gndA [2019-02-19 19:22:38]
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gndA [2019-02-19 19:22:38]

NADP-dependent phosphogluconate dehydrogenase
Locus
BSU23860
Isoelectric point
5.08
Molecular weight
51.61 kDa
Protein length
469 aa Sequence Blast
Gene length
1410 bp Sequence Blast
Function
pentose phosphate pathway
Product
NADP-dependent phosphogluconate dehydrogenase
Essential
no
E.C.
1.1.1.44
Synonyms
yqjI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → Pentose phosphate pathway
  • Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

    • Gene

    Coordinates
    2,480,750 2,482,159

    The protein

    Catalyzed reaction/ biological activity

  • 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH (according to Swiss-Prot)

    • Protein family

  • 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • GntZ

    • Kinetic information

  • Reversible reaction PubMed

    • Modification

  • phosphorylation on (Thr-15 OR Thr-17) PubMed

    • Structure

  • 2W8Z (from Geobacillus stearothermophilus, 83% identity, 92% similarity) PubMed

    • Additional information

  • It contains a cysteine on the active site. The enzyme is a dimer. PubMed
  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    gndA
    Description
    PubMed
    view in new tab

    Genes
    polY1-gndA
    Description
    PubMed

    Regulation

  • constitutively expressed PubMed
    • view in new tab

    Additional information

  • the mRNA is cleaved by RNase III PubMed

    • Biological materials

    Mutant

  • MGNA-C391 (yqjI::erm), available at the NBRP B. subtilis, Japan
  • GP1514 (gndA::kan), available in Jrg Stlke's lab
  • BKE23860 (gndA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCTAACGTCCCTTCT, downstream forward: _UP4_TAATCAATAGAAACCCCCGA
  • BKK23860 (gndA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTCTAACGTCCCTTCT, downstream forward: _UP4_TAATCAATAGAAACCCCCGA

    • Expression vectors

  • pGP1777 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jrg Stlke's lab)
  • pGP1789 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP1389) (available in Jrg Stlke's lab)
  • GP1408 (gndA-Strep (spc)) & GP1410 (gndA-Strep (cat)), purification from B. subtilis, for SPINE, available in Jrg Stlke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab

    • FLAG-tag construct

  • GP1403 (spc, based on pGP1331), available in Jrg Stlke's lab
  • GP1408 (kan, resistance cassette exchange in GP1403), available in Jrg Stlke's lab

    • Antibody

  • **

    • References

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