SubtiBank SubtiBank
floT [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]
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floT [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

membrane-associated scaffold protein, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with YuaF) resistance to cefuroxime
locus
BSU31010
pI
5.00
mw
55.00 kDa
function
involved in the control of membrane fluidity
product
membrane-associated scaffold protein
essential
no
synonyms
yuaG, yuaH

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|biofilm formation], [SW|membrane dynamics], [SW|membrane proteins], [SW|sporulation/ other], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigW regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,180,465 -> 3,181,994

    Phenotypes of a mutant

  • delayed onset of sporulation, reduced sporulation frequency
  • defect in motility [Pubmed|22753055]
  • reduced [SW|protein secretion] [Pubmed|23651456]
  • a ''[protein|search|floT] [protein|search|floA]'' double mutant does not induce [protein|search|KinC]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
  • a ''[protein|search|floT] [protein|search|floA]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
  • a ''[protein|search|floT] [protein|search|floA]'' double mutant has a [SW|sporulation] defect, due to the lack of [protein|search|FtsH] [Pubmed|22882210]
  • a ''[protein|search|floT]'' mutant displays a defective growth under oxygen-limiting conditions [Pubmed|25909364]
  • The protein

    Catalyzed reaction/ biological activity

  • [protein|search|SigW]-dependent expression of ''[protein|search|fabF]'' and the ''[protein|search|yuaF]-[protein|search|floT]-[protein|search|yuaI]'' operon result in reduced membrane fluidity [Pubmed|21542858,22178969]
  • recruits [protein|search|YuaF] to focal assemblies [Pubmed|22753055]
  • controls protease activity of [protein|search|FtsH] [Pubmed|24222488]
  • [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • co-localizes with [protein|search|FloA] and [protein|search|KinC] in discrete foci membrane [Pubmed|20713508]
  • forms discrete focal structures in the cytoplasma membrane [Pubmed|22753055]
  • 6 foci [protein|search|FloT] of are detected in strain 3610 [Pubmed|25909364]
  • [SW|Interactions]

  • forms homo-oligomers [Pubmed|23651456]
  • oligomerization depends on the glutamate-alanine repeats [Pubmed|25909364]
  • [protein|search|YuaF]-[protein|search|FloT] [Pubmed|22753055]
  • [protein|search|FloT]-[protein|search|FtsH] [Pubmed|26297017,22882210]
  • [protein|search|FloT]-[protein|search|FloA] [Pubmed|26297017,23651456]
  • [protein|search|FloT]-[protein|search|SecY] [Pubmed|23651456]
  • [protein|search|FloT]-[protein|search|FtsX] [Pubmed|23651456]
  • [protein|search|FloT]-[protein|search|OppA] [Pubmed|23651456]
  • [protein|search|FloT]-[protein|search|SdhA] [Pubmed|23651456]
  • [protein|search|FloT]-[protein|search|ResE] [Pubmed|26297017,25909364]
  • [protein|search|FloT]-[protein|search|KinC] to stimulate [protein|search|KinC] dimerization and activity [Pubmed|26297017]
  • [protein|search|FloT]-[protein|search|KinD] [Pubmed|26297017]
  • Expression and Regulation

    Operon

  • ''[protein|search|yuaF]-[protein|search|floT]-[protein|search|yuaI]'' [Pubmed|9987136]
  • [SW|Sigma factor]

  • [protein|search|SigW] [Pubmed|9987136]
  • Regulation

  • expressed upon cell wall stress ([protein|search|SigW]) [Pubmed|9987136]
  • repressed by casamino acids [Pubmed|12107147]
  • Biological materials

    Mutant

  • JS152 (markerless), available in [SW|Daniel Lopez]'s lab
  • GFP fusion

  • GK38 (168 ''amyE''::P''floT-yfp'' (spc)), available in [SW|Daniel Lopez]'s lab
  • JS280 (3610 ''amyE::floT-gfp''(spc)), available in [SW|Daniel Lopez]'s lab
  • JS153 (3610 ''lacA::floT''-mEos2 (mls)), available in [SW|Daniel Lopez]'s lab
  • JS166 (3610 ''lacA::floT''-PAmCherry (mls)), available in [SW|Daniel Lopez]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]) for FloT [Pubmed|25909364], available in [SW|Daniel Lopez]'s lab
  • References

    Reviews

  • 25652542
  • Original publications

  • 9987136,22753055,12107147,18763711,19383680,20713508,23651456,22178969,23249255,22882210,25635948,25909364,24222488,26297017,27362352