nusB

transcription antitermination factor

locus: BSU_24320

pI: 4.76

mw: 14.74 kDa

protein length: 131 aa

gene length: 396 bp

function: stable RNA transcription

product: antitermination factor

essential: no

synonyms: yqhZ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.3|Transcription elongation/ termination]

Gene

Coordinates: 2,529,267 2,529,662

The protein

Protein family

• nusB family (single member, according to UniProt)

Structure

• [PDB|1EYV] (from Mycobacterium tuberculosis, 40% identity) [pubmed|10881194]
• [PDB|3R2C] (the [protein|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|NusB]-[protein|751D0B909922D4D931FF3B2285CF142D966FCD48|RpsJ] complex from Aquifex aeolicus) [pubmed|21652641]

Expression and Regulation

Operons

• genes: [gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]-[gene|71FC6E75694B7066D808BAD736140569875E5E5C|folD]
  description: [Pubmed|2536750,11591660]

  regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, (molecular inducer: PRPP) [Pubmed|2536750,11591660], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]
  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • repressed in the presence of purine nucleotides ([protein|search|PurR]) [Pubmed|2536750,11591660]

Biological materials

Mutant

• MGNA-C368 (yqhZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2366 NBRP B. subtilis, Japan]
• BKE24320 ([gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCTCCTTTGAT, downstream forward: _UP4_TAGGAGGAAAGAAAATGACT
• BKK24320 ([gene|6155468AFF216D97373CEF7B8C6E6C7BB9D4617F|nusB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCTTTCTCCTTTGAT, downstream forward: _UP4_TAGGAGGAAAGAAAATGACT

References

• 11948165,16707701,11591660,2536750,10881194,21652641,30023707