ptkA

protein tyrosine kinase

locus: BSU_36250

pI: 9.63

mw: 25.64 kDa

protein length: 237 aa

gene length: 714 bp

function: protein phosphorylation

product: protein tyrosine kinase

essential: no

synonyms: ywqD

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

Gene

Coordinates: 3,731,822 3,732,535

Phenotypes of a mutant

• Accumulation of extra chromosome equivalents [Pubmed|17367396]
• Defect in [SW|biofilm formation], this involves the kinase activity, but the target protein is unknown [Pubmed|20815827]

The protein

Catalyzed reaction/ biological activity

• ATP + L-tyrosyl-[protein] --> ADP + H⁺ + O-phospho-L-tyrosyl-[protein] (according to UniProt)
• autophosphorylation, phosphorylation of [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|Ugd], [protein|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|TuaD], [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB], [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB]

Protein family

• BY-kinase, see the [http://bykdb.ibcp.fr/BYKdb/BYKdbHelp Bacterial Protein Tyrosine Kinase Database]
• CpsD/CapB family (with [protein|9B668909E1380B287ACE58561DCF45FC29184D8B|EpsB], according to UniProt)

Paralogous protein(s)

• [protein|9B668909E1380B287ACE58561DCF45FC29184D8B|EpsB]

[SW|Domains]

• single BY-kinase domain

Modification

• autophosphorylation at residues Y225, Y227 and Y228 (primary site) [Pubmed|20509597], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|PtpZ] [Pubmed|15866923]

[SW|Cofactors]

• ATP

Effectors of protein activity

• [protein|8DCA50478136628DD9E5D01E89609A686EED9F57|TkmA] - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation [Pubmed|12970183]
• the mutually exclusive interactions of PtsA with the modulator proteins [protein|8DCA50478136628DD9E5D01E89609A686EED9F57|TkmA], [protein|8841E35B7A90F6716C0BCD98E8D0CC05FE1CA471|SalA], and probably [protein|37DAD42A391E2FC506225EAF91B8F21629A401DF|MinD] direct the kinase to different substrates [Pubmed|27725816]

Structure

• [PDB|2VED] (CapB, the homolog in ''Staphylococcus aureus'')

Expression and Regulation

Operons

• genes: [gene|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]-[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]-[gene|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd]
  description: [Pubmed|20815827,12970183]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20815827], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|26283769], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|26283769], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• MGNA-A078 (ywqD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/78 NBRP B. subtilis, Japan]
• KO strain created with pMUTIN-2, available from [SW|Ivan Mijakovic]
• GP1520 (spc), available in [SW|Jörg Stülke]'s lab
• GP1544 (ermC), available in [SW|Jörg Stülke]'s lab
• GP1587 (cat) , available in [SW|Jörg Stülke]'s lab
• GP1521 ''[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]'' (aphA3) ''[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]'' (spc) double mutant available in [SW|Jörg Stülke]'s lab
• GP1529 ''[gene|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]-[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]''::spc available in [SW|Jörg Stülke]'s lab
• GP1610 (''[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]'', spc), available in [SW|Jörg Stülke]'s lab
• BKE36250 ([gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCATCCGCGAGCCTCTGT, downstream forward: _UP4_TAAATAACGTGCATCGTGCC
• BKK36250 ([gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCATCCGCGAGCCTCTGT, downstream forward: _UP4_TAAATAACGTGCATCGTGCC

Expression vectors

• pQE-30, N-terminally 6xHis-tagged, available from [SW|Ivan Mijakovic]

lacZ fusion

• in a KO strain created with pMUTIN-2, available from [SW|Ivan Mijakovic]

GFP fusion

• CFP-fusion, available from [SW|Ivan Mijakovic]

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

labs

• [SW|Ivan Mijakovic], Thiverval-Grignon, France

References

Reviews

• 20497498,24554699,25540643,25667587,26286503

Original publications

• 12970183,15741737,15866923,17367396,19258708,18547145,20497499,20509597,20815827,20817675,23939619,24493247,24728941,25278935,25374563,26283769,26094643,27148221,27725816