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function
protein phosphorylation
product
protein tyrosine kinase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]Gene
Coordinates
3,731,822 3,732,535
Phenotypes of a mutant
Accumulation of extra chromosome equivalents [Pubmed|17367396]Defect in [SW|biofilm formation], this involves the kinase activity, but the target protein is unknown [Pubmed|20815827]The protein
Catalyzed reaction/ biological activity
ATP + L-tyrosyl-[protein] --> ADP + H+ + O-phospho-L-tyrosyl-[protein] (according to UniProt)autophosphorylation, phosphorylation of [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|Ugd], [protein|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|TuaD], [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB], [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB]Protein family
BY-kinase, see the [http://bykdb.ibcp.fr/BYKdb/BYKdbHelp Bacterial Protein Tyrosine Kinase Database]CpsD/CapB family (with [protein|9B668909E1380B287ACE58561DCF45FC29184D8B|EpsB], according to UniProt)Paralogous protein(s)
[protein|9B668909E1380B287ACE58561DCF45FC29184D8B|EpsB] [SW|Domains]
single BY-kinase domainModification
autophosphorylation at residues Y225, Y227 and Y228 (primary site) [Pubmed|20509597], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|PtpZ] [Pubmed|15866923][SW|Cofactors]
ATPEffectors of protein activity
[protein|8DCA50478136628DD9E5D01E89609A686EED9F57|TkmA] - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation [Pubmed|12970183]the mutually exclusive interactions of PtsA with the modulator proteins [protein|8DCA50478136628DD9E5D01E89609A686EED9F57|TkmA], [protein|8841E35B7A90F6716C0BCD98E8D0CC05FE1CA471|SalA], and probably [protein|37DAD42A391E2FC506225EAF91B8F21629A401DF|MinD] direct the kinase to different substrates [Pubmed|27725816]Structure
[PDB|2VED] (CapB, the homolog in ''Staphylococcus aureus'')Expression and Regulation
Operons
genes
[gene|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]-[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]-[gene|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|ugd]
description
[Pubmed|20815827,12970183]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20815827], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|26283769], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon][protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|26283769], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon][protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]view in new tabBiological materials
Mutant
MGNA-A078 (ywqD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/78 NBRP B. subtilis, Japan]KO strain created with pMUTIN-2, available from [SW|Ivan Mijakovic]GP1520 (spc), available in [SW|Jörg Stülke]'s labGP1544 (ermC), available in [SW|Jörg Stülke]'s labGP1587 (cat) , available in [SW|Jörg Stülke]'s labGP1521 ''[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]'' (aphA3) ''[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]'' (spc) double mutant available in [SW|Jörg Stülke]'s labGP1529 ''[gene|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]-[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]''::spc available in [SW|Jörg Stülke]'s labGP1610 (''[gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]-[gene|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ]'', spc), available in [SW|Jörg Stülke]'s labBKE36250 ([gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCATCCGCGAGCCTCTGT, downstream forward: _UP4_TAAATAACGTGCATCGTGCCBKK36250 ([gene|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCATCCGCGAGCCTCTGT, downstream forward: _UP4_TAAATAACGTGCATCGTGCCExpression vectors
pQE-30, N-terminally 6xHis-tagged, available from [SW|Ivan Mijakovic]lacZ fusion
in a KO strain created with pMUTIN-2, available from [SW|Ivan Mijakovic]GFP fusion
CFP-fusion, available from [SW|Ivan Mijakovic]two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lablabs
[SW|Ivan Mijakovic], Thiverval-Grignon, FranceReferences
Reviews
20497498,24554699,25540643,25667587,26286503 Original publications
12970183,15741737,15866923,17367396,19258708,18547145,20497499,20509597,20815827,20817675,23939619,24493247,24728941,25278935,25374563,26283769,26094643,27148221,27725816