bshB1 [2019-02-19 19:22:30]

N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis (major enzyme)

locus: BSU22470

pI: 6.34

mw: 24.66 kDa

protein length: 236 aa

gene length: 711 bp

function: biosynthesis of bacillithiol

product: N-acetylglucosamin-malate deacetylase

essential: no

synonyms: jojG,ypjG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.2|Biosynthesis of bacillithiol]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,358,208 2,358,918

The protein

Catalyzed reaction/ biological activity

• GlcNAc-Mal GlcN-Mal + Ac

Paralogous protein(s)

• [protein|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|BshB2]

Structure

• [PDB|2IXD] (from B. cereus, 62% identity) [pubmed|17501983]

Expression and Regulation

Operons

• genes: [gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
  description: [Pubmed|20308541]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

Biological materials

Mutant

• MGNA-A409 (ypjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/409 NBRP B. subtilis, Japan]
• ''bshB1'' null mutant and ''[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]'' / ''[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]'' double null mutant available in [SW|John Helmann] lab
• GP87 (''ypjG''::''pX2''(''cat'')), available in [SW|Stlke] lab
• BKE22470 ([gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG
• BKK22470 ([gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG

References

Reviews

• 28117687

Original Publications

• 20308541,19578333,23894131,23758290,25988368,17501983