bshB1 [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis (major enzyme)

locus: BSU22470

pI: 6.34

mw: 24.00 kDa

function: biosynthesis of bacillithiol

product: N-acetylglucosamin-malate deacetylase

essential: no

synonyms: jojG, ypjG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.2|Biosynthesis of bacillithiol]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

[SW|Categories] containing this gene/protein

• [SW|miscellaneous metabolic pathways], [SW|resistance against oxidative and electrophile stress]

This gene is a member of the following [SW|regulons]

• [SW|Spx regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,358,208 -> 2,358,918

The protein

Catalyzed reaction/ biological activity

• GlcNAc-Mal -> GlcN-Mal + Ac

Paralogous protein(s)

• [SW|BshB2]

Expression and Regulation

Operon

• ''[protein|search|ypjD]-[protein|search|dapB]-[protein|search|mgsA]-[SW|bshB1]-[protein|search|bshA]-[protein|search|cca]-[protein|search|birA]'' [Pubmed|20308541]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|23894131]

Regulation

• induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

Regulatory mechanism

• [protein|search|Spx]: transcription activation [Pubmed|23894131]

Biological materials

Mutant

• ''bshB1'' null mutant and ''[SW|bshB1]'' / ''[SW|bshB2]'' double null mutant available in [SW|John Helmann] lab
• GP87 (''ypjG''::''pX2''(''cat'')), available in [SW|Stülke] lab

References

• 20308541,19578333,23894131,23758290,25988368