bshB1 [2018-02-09T22:46:47.000Z]

N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis (major enzyme)

locus: BSU22470

pI: 6.34

mw: 24.66 kDa

protein length: 224 aa

gene length: 672 bp

function: biosynthesis of bacillithiol

product: N-acetylglucosamin-malate deacetylase

essential: no

synonyms: jojG,ypjG

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.2|Biosynthesis of bacillithiol]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 2,358,208 → 2,358,918

The protein

Catalyzed reaction/ biological activity

• GlcNAc-Mal → GlcN-Mal + Ac

Paralogous protein(s)

• [protein|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|BshB2]

Structure

• [PDB|2IXD] (from B. cereus, 62% identity) [pubmed|17501983]

Biological materials

Mutant

• MGNA-A409 (ypjG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/409 NBRP B. subtilis, Japan]
• ''bshB1'' null mutant and ''[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]'' / ''[gene|8106404D9D2CFA0825E1284D47CC7198D8A20E5D|bshB2]'' double null mutant available in [SW|John Helmann] lab
• GP87 (''ypjG''::''pX2''(''cat'')), available in [SW|Stülke] lab
• BKE22470 (Δ[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG
• BKK22470 (Δ[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT, downstream forward: _UP4_CTTGATCATGATGTGCTTGG

References

Reviews

• 28117687

Original Publications

• 20308541,19578333,23894131,23758290,25988368,17501983