SubtiBank SubtiBank
spoIIIAA
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

spoIIIAA

AAA protease, required for [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG] activation
locus
BSU_24430
pI
8.18
mw
34.14 kDa
protein length
307 aa Sequence Blast
gene length
924 bp Sequence Blast
function
activation of [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]
product
AAA protease
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    2,536,690 2,537,613

    The protein

    [SW|Localization]

  • membrane protein [Pubmed|18485064]
  • Expression and Regulation

    Operons

    genes
    [gene|70408C17CF8681998F7FD933D4BFF71D052626D9|yqhV]-[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
    description
    [Pubmed|1766372]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,18485064], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: repression, [Pubmed|15383836], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]
  • regulation

  • both promoters: expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|18485064], switched off by [SW|SpoIIID] [Pubmed|15383836]
  • additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of [protein|search|spoIIIAA], suggesting that [protein|search|SpoIIIAG] and [protein|search|SpoIIIAH] are required in larger amounts as compared to the other products of the operon [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed]
  • view in new tab

    genes
    [gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
    description
    [Pubmed|18485064]

    regulation

  • both promoters: expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|18485064], switched off by [SW|SpoIIID] [Pubmed|15383836]
  • additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of [protein|search|spoIIIAA], suggesting that [protein|search|SpoIIIAG] and [protein|search|SpoIIIAH] are required in larger amounts as compared to the other products of the operon [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed]
  • view in new tab

    Biological materials

    Mutant

  • BKE24430 ([gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACAGAGCCTCCTCCTTTC , downstream forward: _UP4_AATGTGCTGTCTAGGACGACG
  • BKK24430 ([gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACAGAGCCTCCTCCTTTC , downstream forward: _UP4_AATGTGCTGTCTAGGACGACG
  • References

    Reviews

  • 31350897
  • Original Publications

  • 1766372,18485064