SubtiBank SubtiBank
yrkF
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yrkF

putative sulfur carrier protein
locus
BSU_26530
pI
5.99
mw
20.51 kDa
protein length
185 aa Sequence Blast
gene length
558 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]
  • Gene

    Coordinates
    2,712,577 2,713,134

    The protein

    Protein family

  • sulfur carrier protein TusA family (with [protein|D996F581E21395C3F9CC064800C49A765EFC17A7|YrkI], according to UniProt)
  • [SW|Domains]

  • [SW|Rhodanese domain] (aa 101-185) (according to UniProt)
  • Structure

  • [PDB|1DCJ] (E. coli TusA, corresponds to the N-terminal domain of [protein|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|YrkF], aa 8 ... 77, 37% identity)
  • [PDB|3R2U] (metallo--lactamase from Staphylococcus aureus, corresponds to the C-terminal domain of [protein|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|YrkF], aa 106 ... 179, 38% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|6AD1DD074B2F044C6A4E4B98FABC4E7FD67B3139|yrzM]-[gene|8A3F1BFDF85DD96B7FB4EBC807F71639AF69DE98|yrkD]-[gene|1F1FE531622CD218EE8990CDAC6D2D2F9E86FE3F|yrkE]-[gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]-[gene|06CC915A18E5ADD42B784B7BBBFD4C344559A34E|yrkH]-[gene|D996F581E21395C3F9CC064800C49A765EFC17A7|yrkI]-[gene|F3E842844B315FE438F66995CDFB8BA1C32BDF23|yrkJ]-[gene|1BEBD09273240B6B50DABCB160FCCBC205BB2AAE|yrkK]-[gene|search|yrkM]
    description
    [Pubmed|22383849]
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    genes
    [gene|6AD1DD074B2F044C6A4E4B98FABC4E7FD67B3139|yrzM]-[gene|8A3F1BFDF85DD96B7FB4EBC807F71639AF69DE98|yrkD]-[gene|1F1FE531622CD218EE8990CDAC6D2D2F9E86FE3F|yrkE]-[gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]-[gene|06CC915A18E5ADD42B784B7BBBFD4C344559A34E|yrkH]-[gene|D996F581E21395C3F9CC064800C49A765EFC17A7|yrkI]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • BKE26530 ([gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCCTCCTTTTCTT, downstream forward: _UP4_TAAATAAAAAGCATGATGAA
  • BKK26530 ([gene|5FC3DF596816AA4E8541B324EC6DE6D89C90C8E2|yrkF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTCCTCCTTTTCTT, downstream forward: _UP4_TAAATAAAAAGCATGATGAA