queF

nitrile reductase (NADPH-dependent 7-cyano-7-deazaguanine reductase), synthesis of the modified ribonucleotide queuosine

locus: BSU_13750

pI: 4.93

mw: 19.23 kDa

protein length: 165 aa

gene length: 498 bp

function: tRNA modification

product: nitrile reductase

essential: no

ec: 1.7.1.13

synonyms: ykvM

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]

Gene

Coordinates: 1,441,291 1,441,788

The protein

Catalyzed reaction/ biological activity

• 7-aminomethyl-7-carbaguanine + 2 NADP⁺ --> 7-cyano-7-deazaguanine + 3 H⁺ + 2 NADPH (according to UniProt)

Protein family

• GTP cyclohydrolase I family (with [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|FolE], according to UniProt)

Modification

• active site Cys55 is S-bacillithiolated by NaOCl stress [Pubmed|22938038]
• disulfide formation between the catalytic Cys55 and conserved Cys99 protects the protein from irreversible oxidation [pubmed|28300774]

[SW|Cofactors]

• NADPH [pubmed|28300774]

Structure

• [PDB|4F8B] (covalent thioimide intermediate of the unimodular nitrile reductase [protein|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|QueF]) [Pubmed|22787148]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]-[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]-[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]-[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]
  description: [Pubmed|14660578]

  regulatory mechanism

  • [regulon|preQ1 riboswitch|preQ1 riboswitch]: antitermination, in the absence of queuosine [Pubmed|19285444], in [regulon|preQ1 riboswitch|preQ1 riboswitch]

  regulation

  • repressed in the presence of queuosine ([SW|preQ1 riboswitch]) [Pubmed|19285444]

Biological materials

Mutant

• MGNA-B326 (ykvM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1325 NBRP B. subtilis, Japan]
• BKE13750 ([gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTCATGTTCATCTTCCTTT, downstream forward: _UP4_TAATTATTCCGTTGTGTGAT
• BKK13750 ([gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTCATGTTCATCTTCCTTT, downstream forward: _UP4_TAATTATTCCGTTGTGTGAT

References

• 22938038,14660578,15767583,16511203,17929836,19285444,17384645,22787148,28300774,28516784