sigG [2020-07-30 15:30:06]

[SW|RNA polymerase] [SW|sporulation] forespore-specific (late) [SW|sigma factor] SigG

locus: BSU_15330

pI: 5.50

mw: 29.92 kDa

protein length: 260 aa

gene length: 783 bp

function: transcription of [SW|sporulation] genes (late forespore)

product: [SW|RNA polymerase] [SW|sporulation] forespore-specific (late) [SW|sigma factor] SigG

essential: no

synonyms: spoIIIG

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 1,605,630 1,606,412

The protein

Protein family

• [SW|Sigma-70 factor family] (according to UniProt)

Paralogous protein(s)

• [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]

Effectors of protein activity

• [protein|01412FA454DDBA12622E3B32C433F8059123661B|CsfB] inhibits SigG activity [Pubmed|19497328]
• the activity of [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]-containing [SW|RNA polymerase] is modulated by [protein|8AB50581EBB3C27BB9A96EA3382F8C90CE5B6EC5|YlyA] [Pubmed|23678950]

Structure

• [PDB|1L0O] ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] in complex with [protein|C8B867758330355F44C63CC284DC7AA3061FB9F1|SpoIIAB], Geobacillus stearothermophilus, 47% identity) [pubmed|11955433]

Biological materials

Mutant

• BKE15330 ([gene|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTTCCCTCCCTACA, downstream forward: _UP4_TAATGAAAAGCCTTTAAAAC
• BKK15330 ([gene|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTTTCCCTCCCTACA, downstream forward: _UP4_TAATGAAAAGCCTTTAAAAC

labs

• [SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

References

Reviews

• 20833318,31350897,1

The [SW|SigG regulon]

• 16497325

Original Publications

• 1900507,1902463,15175314,10806362,1902213,2497051,12867473,16707705,9829949,1487728,2448286,9852018,19497328,8126438,8355618,24163345,18820020,2493633,7961403,15256570,12813085,9004507,8885263,18485064,19390092,23678950,22984259,20817675,21935351,25835496,29702640,11955433