fabF

beta-ketoacyl-acyl carrier protein synthase II, involved in the control of membrane fluidity

locus: BSU_11340

pI: 4.77

mw: 43.84 kDa

protein length: 413 aa

gene length: 1242 bp

function: fatty acid biosynthesis

product: beta-ketoacyl-acyl carrier protein synthase II

essential: yes

ec: 2.3.1.179

synonyms: yjaY

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 1,209,183 1,210,424

Phenotypes of a mutant

• essential [Pubmed|12682299]
• CRISPRi knock-down: terminal phenotype of growth cessation, moderate bending [Pubmed|27238023]
• FabF-I109F: resistance to cerulenin [Pubmed|11325930,24641521]
• FabF-L112F, FabF-I109M: resistance to cerulenin (Zeigler DR, unpublished observations)

The protein

Catalyzed reaction/ biological activity

• (11Z)-hexadecenoyl-[ACP] + H⁺ + malonyl-[ACP] --> 3-oxo-(13Z)-octadecenoyl-[ACP] + CO₂ + holo-[ACP](according to UniProt)
• [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]-dependent expression of ''[gene|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF]'' and the ''[gene|FFBB9B236B7D532D45FE0593B793E28E051BE7A2|yuaF]-[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]-[gene|56345EA776E1C5664EF1F2D1AF5CE3E5F40AD442|yuaI]'' operon result in reduced membrane fluidity [Pubmed|21542858,22178969]

Protein family

• [SW|thiolase-like superfamily] (according to UniProt)

Structure

• [PDB|4LS5] [Pubmed|24641521]

Expression and Regulation

Operons

• genes: [gene|40E064365588CFED483FBFED02BFC0DA531CCC4A|fabHA]-[gene|5D5D9A544295DD9B55C75A8CF47AB19935E740C6|fabF]
  description: [Pubmed|12737802]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12737802], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|21542858], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulatory mechanism

  • [protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]: repression, [Pubmed|12737802], in [regulon|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR regulon]

  regulation

  • ''[SW|fabHA]'': expressed when the cells experience a lack of malonyl-CoA ([protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]) [Pubmed|12737802]
  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• BEC11340 (lacA::Pxyl-dcas9 amyE::Pveg-sgRNA(fabF) trpC2) [http://www.bgsc.org/getdetail.php?bgscid=BEC11340 BGSC], [Pubmed|27238023]
• 1A573 (fabF2 trpC2) [http://www.bgsc.org/getdetail.php?bgscid=1A573 BGSC], cerulenin-resistant mutant
• 1A576 (fabF14 trpC2) [http://www.bgsc.org/getdetail.php?bgscid=1A576 BGSC], cerulenin-resistant mutant
• 1A577 (fabF20 trpC2) [http://www.bgsc.org/getdetail.php?bgscid=1A577 BGSC], cerulenin-resistant mutant

Expression vector

• pGP3509 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
• pGP3510: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [https://www.thermofisher.com/order/catalog/product/K30001#/K30001 pETSUMOadapt], available in [SW|Jörg Stülke]'s lab
• pGP3511: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab

References

Reviews

• 15952903,17919287

Original Publications

• 11325930,18384517,12837788,12737802,12682299,21383089,24641521,21542858,22178969,24641521,27238023