yjcG [2019-08-22 10:17:09]

2´-CPDase

locus: BSU_11850

pI: 5.57

mw: 19.52 kDa

protein length: 171 aa

gene length: 516 bp

function: hydrolysis of 2´,3´-cyclic nucleotides

product: 2´-CPDase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.4|Nucleotide metabolism/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,256,862 1,257,377

The protein

Catalyzed reaction/ biological activity

• hydrolyzes a 2´,3´-cyclic nucleotide, thereby producing a nucleotide with a 3´-phosphate [pubmed|31422053]

Protein family

• 2H phosphoesterase superfamily (with [protein|29A38D244A07ED0973D0846B652238524F4DBC67|YtlP], according to UniProt) [pubmed|31422053]

Paralogous protein(s)

• [protein|29A38D244A07ED0973D0846B652238524F4DBC67|YtlP]

Structure

• [PDB|2D4G]

Expression and Regulation

Operons

• genes: [gene|94EE92D99CA68856E6D19254B0768FFE92AF603D|yjcH]-[gene|5CA17CD28A725B8D7B87B9AAA167844042439794|yjcG]-[gene|5E4C3ADF5B5D0215E9E350F16BCA57B208933606|yjcF]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B255 (yjcG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1254 NBRP B. subtilis, Japan]
• BKE11850 ([gene|5CA17CD28A725B8D7B87B9AAA167844042439794|yjcG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGTCCCTCCTGGA, downstream forward: _UP4_TTGCTAGGCAGAGGAGAATA
• BKK11850 ([gene|5CA17CD28A725B8D7B87B9AAA167844042439794|yjcG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATGTCCCTCCTGGA, downstream forward: _UP4_TTGCTAGGCAGAGGAGAATA

References

• 12107147,18473364,16511078,31422053