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hisI
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hisI

phosphoribosyl-AMP cyclohydrolase / phosphoribosyl-ATP pyrophosphohydrolase
Locus
BSU_34860
Isoelectric point
4.63
Molecular weight
23.75 kDa
Protein length
209 aa Sequence Blast
Gene length
630 bp Sequence Blast
Function
biosynthesis of histidine
Product
phosphoribosyl-AMP cyclohydrolase /phosphoribosyl-ATP pyrophosphohydrolase
Essential
no
E.C.
3.5.4.19
Synonyms
hisIE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of histidine

    • Gene

    Coordinates
    3,582,936 3,583,565

    The protein

    Catalyzed reaction/ biological activity

  • 1-(5-phospho-β-D-ribosyl)-ATP + H2O --> 1-(5-phospho-β-D-ribosyl)-5'-AMP + diphosphate + H+ (according to UniProt)
  • 1-(5-phospho-β-D-ribosyl)-5'-AMP + H2O --> 1-(5-phospho-β-D-ribosyl)-5-[(5-phospho-β-D-ribosylamino)methylideneamino]imidazole-4-carboxamide (according to UniProt)

    • Domains

  • N-terminal domain (aa 1 ... 116): phosphoribosyl-AMP cyclohydrolase domain (according to UniProt) PubMed
  • C-terminal domain (aa 117 ... 209): phosphoribosyl-ATP pyrophosphohydrolase domain (according to UniProt)

    • Structure

  • 1ZPS (the N-terminal domain, from Methanobacterium thermoautotrophicum, 49% identity)
  • 1YVW (the C-terminal domain, from B. cereus, 49% identity)

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI
    Description
    PubMed

    Regulatory mechanism

  • YlxR: regulation, PubMed, in YlxR regulon

    • Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
  • expression depends on functional YlxR PubMed
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    Biological materials

    Mutant

  • BKE34860 (hisI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAAACGCAGTTCATCTGCCT, downstream forward: _UP4_TAAAGAGCCGGATGATACGG
  • BKK34860 (hisI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AAAACGCAGTTCATCTGCCT, downstream forward: _UP4_TAAAGAGCCGGATGATACGG

    • References

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