tmk

thymidylate kinase

locus: BSU_00280

pI: 4.87

mw: 23.73 kDa

protein length: 212 aa

gene length: 639 bp

function: TTP biosynthesis

product: thymidylate kinase

essential: yes

ec: 2.7.4.9

synonyms: yaaP

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 39,159 39,797

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + dTMP --> ADP + dTDP (according to UniProt)

Protein family

• Thymidylate kinase family (single member, according to UniProt)

Structure

• [PDB|2CCK] (from ''Staphylococcus aureus'', 53% identity, 74% similarity) [Pubmed|16522804]

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|08314FA6195D553B8522BCD3B45FEE0EAB51F64D|xpaC]-[gene|5231A0EE9C5A7556817B686D5B2B7B72B675BCAC|yaaN]-[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]-[gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
  description: [Pubmed|22383849]

• genes: [gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|yaaT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
  description: [Pubmed|22383849]

Biological materials

Expression vectors

• pGP1689: expression of Strep-''tmk'' in ''E. coli'', in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
• pGP2671: expression of ''tmk''-Strep, in [SW|pGP380] ,in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP2401 ''tmk-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

• 16522804,18523102,23043329,18523102,27376462,23394555