comP

two-component sensor kinase, detects extracellular [protein|60D6EB02923D9ADE88F61A8CBBD882BA8BDD457E|ComX]

locus: BSU_31690

pI: 8.00

mw: 89.13 kDa

protein length: 769 aa

gene length: 2310 bp

function: regulation of genetic competence and quorum sensing

product: two-component sensor kinase

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 3,253,529 3,255,838

Phenotypes of a mutant

• the mutation suppresses the mucoid phenotype of ''[gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]'' or ''[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]'' mutants due to loss of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] phosphorylation and concomitant reduced expression of the ''[gene|86C05126ADBA22BB1771B1FA3D214B2E7A36311E|capB]-[gene|D66994D077D1382B88FF65075E44C2A31089548F|capC]-[gene|A696434A086A428D411CAF45B37CD8F82AC2503F|capA]-[gene|6B61DCE8D27776EBF942621D3AE90F410FD13F37|capE]'' operon [Pubmed|24296669]

The protein

Catalyzed reaction/ biological activity

• autophosphorylation
• phosphorylation of [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] when bound to [protein|60D6EB02923D9ADE88F61A8CBBD882BA8BDD457E|ComX]
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

[SW|Domains]

• eight transmembrane segments
• [SW|Histidine kinase domain] (aa 571-769) (according to UniProt)

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• [protein|60D6EB02923D9ADE88F61A8CBBD882BA8BDD457E|ComX] (extracellular)

[SW|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|FDACEF9DDC3BEA4EB013AE67BC47ADA532F00205|comQ]-[gene|60D6EB02923D9ADE88F61A8CBBD882BA8BDD457E|comX]-[gene|5A00E233F0B5A0DAB18D2ECC55527F1911987F86|comP]-[gene|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]-[gene|015989AAB862E418C944AB35DA3605863F059CE9|yuxO]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2507523], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• BKE31690 ([gene|5A00E233F0B5A0DAB18D2ECC55527F1911987F86|comP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATATTAATCCACCTATTA, downstream forward: _UP4_TAATGGATTTATAACGGAAA
• BKK31690 ([gene|5A00E233F0B5A0DAB18D2ECC55527F1911987F86|comP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATATTAATCCACCTATTA, downstream forward: _UP4_TAATGGATTTATAACGGAAA

References

Reviews

• 12576575,30218468

Original Publications

• 11133937,8643670,12067344,10094672,10419951,2116363,9274030,18763711,24296669,24130811,24425772,24788106,25757097,26787913,26927849,29038467