SubtiBank SubtiBank
prfA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

prfA

peptide chain release factor 1
locus
BSU37010
pI
4.82
mw
40.07 kDa
protein length
356 aa Sequence Blast
gene length
1068 bp Sequence Blast
function
translation
product
peptide chain release factor 1
essential
yes
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,797,085 → 3,798,155

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfA is highly specific for a U in the first stop-codon position and recognizes UAA and UAG
  • Protein family

  • prokaryotic/mitochondrial release factor family (according to Swiss-Prot)
  • Structure

  • [PDB|1ZBT] (from ''Streptococcus mutans'', 58% identity)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • References

  • 12237459,20512119,25217634