opuD

glycine betaine and arsenobetaine transporter

locus: BSU_30070

pI: 9.51

mw: 55.95 kDa

protein length: 512 aa

gene length: 1539 bp

function: compatible solute transport

product: glycine betaine transporter

essential: no

synonyms: ytfQ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.9|Uptake of compatible solutes]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,076,818 3,078,356

The protein

Catalyzed reaction/ biological activity

• uptake of glycine betaine and arsenobetaine [pubmed|29159878]

Protein family

• BCCT transporter (TC 2.A.15) family (single member, according to UniProt)

Structure

• [PDB|4AIN] (BetP from Corynebacterium glutamicum, 44% identity) [pubmed|22940865]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|86274AAF9E0D0882F1AD9D4A528BECB013B71C38|ytfP]-[gene|597B517CA4277E33153F80919213BFF7BE495563|opuD]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21296969], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|21296969], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by cold stress [Pubmed|21296969]

Biological materials

Mutant

• MGNA-A815 (opuD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/815 NBRP B. subtilis, Japan]
• 1A1052 ( ''opuD''::''kan''), [Pubmed|21296969], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1052&Search=1A1052 BGSC]
• 1A1053 ( ''opuD''::''kan''), [Pubmed|21296969], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1053&Search=1A1053 BGSC]
• 1A1054 ( ''opuD''::''kan''), [Pubmed|21296969], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1054&Search=1A1054 BGSC]
• 1A1055 ( ''opuD''::''kan''), [Pubmed|21296969], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1055&Search=1A1055 BGSC]
• BKE30070 ([gene|597B517CA4277E33153F80919213BFF7BE495563|opuD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTCACCCTCTAATTT, downstream forward: _UP4_TAACAAAAAAGATCTTTCCG
• BKK30070 ([gene|597B517CA4277E33153F80919213BFF7BE495563|opuD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTCACCCTCTAATTT, downstream forward: _UP4_TAACAAAAAAGATCTTTCCG

labs

• [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

References

Reviews

• 27935846

Original publications

• 8752321,21296969,8752321,29159878,22940865