SubtiBank SubtiBank
phoD [Thu Oct 27 2016 11:12:00 GMT+0200 (CEST)]
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phoD [Thu Oct 27 2016 11:12:00 GMT+0200 (CEST)]

phosphodiesterase/alkaline phosphatase, degrades wall teichoic acid during phosphate starvation
locus
BSU02620
pI
8.00
mw
62.00 kDa
function
aquisition of phosphate upon phosphoate starvation, degradation of wall teichoic acid during phosphate starvation
product
phosphodiesterase/alkaline phosphatase
essential
no
ec
3.1.3.1
synonyms
ycbS

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|phosphate metabolism]
  • This gene is a member of the following [SW|regulons]

  • [SW|PhoP regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    284,011 -> 285,762

    The protein

    Catalyzed reaction/ biological activity

  • endohydrolytic activity on wall teichoic acid, acts in concert with [SW|GlpQ] [Pubmed|27780866]
  • A phosphate monoester H2O = an alcohol phosphate (according to Swiss-Prot)
  • [SW|Cofactors]

  • one Fe3 and two Ca2 [Pubmed|25217636]
  • Structure

  • [PDB|2YEQ] [Pubmed|25217636]
  • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862], secreted by the [SW|TatAD]-[SW|TatCD] complex [Pubmed|19395490]
  • [SW|Interactions]

  • [SW|TatAD]-[protein|search|PhoD] [Pubmed|12867413]
  • Expression and Regulation

    Operon

  • ''[protein|search|phoD]-[SW|tatAD]-[SW|tatCD]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|10094677]
  • Regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|10094677]
  • Regulatory mechanism

  • [protein|search|PhoP]: transcription activation [Pubmed|10094677]
  • Additional information

  • expression of the operon depends on functional [protein|search|HemAT] and [protein|search|CsbC] [PubMed|23180473]
  • Labs working on this gene/protein

  • [SW|Oscar Kuipers], University of Groningen, The Netherlands
  • [http://molgen.biol.rug.nl/molgen/index.php Homepage]
  • References

    Reviews

  • 24140208
  • Original publications

  • 8760916,10913081,12867413,10094677,12218047,10556724,19180538,10094677,18957862,19383693,19395490,22960285,22383849,23180473,25217636,25666134,27118079,27780866