phoD

phosphodiesterase/alkaline phosphatase, degrades wall teichoic acid during phosphate starvation

locus: BSU_02620

pI: 8.39

mw: 62.66 kDa

protein length: 583 aa

gene length: 1752 bp

function: aquisition of phosphate upon phosphoate starvation, degradation of wall teichoic acid during phosphate starvation

product: phosphodiesterase/alkaline phosphatase

essential: no

ec: 3.1.3.1

synonyms: ycbS

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 284,011 285,762

The protein

Catalyzed reaction/ biological activity

• endohydrolytic activity on wall teichoic acid, acts in concert with [protein|E9BEF368F8E55888AD4849E8906E517831A882DF|GlpQ] [Pubmed|27780866]
• phosphate monoester + H₂O --> alcohol + phosphate (according to UniProt)

Protein family

• PhoD family (single member, according to UniProt)

[SW|Cofactors]

• one Fe³⁺ and two Ca²⁺ [Pubmed|25217636]

Structure

• [PDB|2YEQ] [Pubmed|25217636]

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862], secreted by the [protein|E4395255CD43ACB611E0BA872182DF801662C366|TatAD]-[protein|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|TatCD] complex [Pubmed|19395490]

Expression and Regulation

Operons

• genes: [gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]-[gene|E4395255CD43ACB611E0BA872182DF801662C366|tatAD]-[gene|A58C9B9BB6574662A44AF0C7A94DFFE368B740E2|tatCD]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10094677], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|10094677,11007775], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]

  regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|10094677]

  additional information

  • expression of the operon depends on functional [protein|search|HemAT] and [protein|search|CsbC] [PubMed|23180473]

Biological materials

Mutant

• BKE02620 ([gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGATCCCCTCTCTC, downstream forward: _UP4_ATCACGAATTAAGGAGTGGA
• BKK02620 ([gene|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGATCCCCTCTCTC, downstream forward: _UP4_ATCACGAATTAAGGAGTGGA

labs

• [SW|Oscar Kuipers], University of Groningen, The Netherlands
• [http://molgen.biol.rug.nl/molgen/index.php Homepage]

References

Reviews

• 24140208

Original publications

• 8760916,10913081,12867413,10094677,12218047,10556724,19180538,10094677,18957862,19383693,19395490,22960285,22383849,23180473,25217636,25666134,27118079,27780866