ytpB

tetraprenyl-beta-curcumene synthase, catalyzes the first committed step in C35 terpenoid biosynthesis, salt stress protein

locus: BSU_30500

pI: 5.76

mw: 42.68 kDa

protein length: 367 aa

gene length: 1104 bp

function: C35 terpenoid biosynthesis

product: tetraprenyl-beta-curcumene synthase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates: 3,121,538 3,122,641

Phenotypes of a mutant

• increased sensitivity to bacitracin, due to the accumulation of heptaprenyl pyrophosphate [Pubmed|24806199]
• sensitivity is increased in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB] [gene|34DBBDD76A0B36867E5116FF17391DF70062F745|menA]'' double mutant [Pubmed|24806199]

The protein

Catalyzed reaction/ biological activity

• all-trans-heptaprenyl diphosphate --> diphosphate + tetraprenyl-β-curcumene (according to UniProt)

Structure

• [PDB|5YO8] (from Bacillus alcalophilus, 50% identity)

Expression and Regulation

Operons

• genes: [gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]
  description: [Pubmed|9387221]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulation

  • expressed under conditions of salt stress ([protein|search|SigM]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]

• genes: [gene|85A653AF4752A61A39F9C42FC791B2D1444886B1|ytpA]-[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]
  description: [Pubmed|18179421]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulation

  • expressed under conditions of salt stress ([protein|search|SigM]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]

Biological materials

Mutant

• MGNA-A164 (ytpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/164 NBRP B. subtilis, Japan]
• BKE30500 ([gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACACATGCACCCCCTACT, downstream forward: _UP4_AAAAATTCAAAGAAAAAAGC
• BKK30500 ([gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACACATGCACCCCCTACT, downstream forward: _UP4_AAAAATTCAAAGAAAAAAGC

References

• 9387221,18179421,23748782,21627333,24806199