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ydaO [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
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ydaO [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

putative transporter
locus
BSU04320
pI
9.00
mw
66.00 kDa
function
unknown
product
unknown
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [SW|Categories] containing this gene/protein

  • [SW|membrane proteins], [SW|proteins of unknown function]
  • This gene is a member of the following [SW|regulons]

  • [SW|YdaO riboswitch]
  • Gene

    Coordinates on the chromosome (coding sequence)
    486,432 -> 488,255

    The protein

    [SW|Localization]

  • cell membrane (according to [http://www.uniprot.org/uniprot/P96589 UniProt])
  • Expression and Regulation

    Operon

  • ''[protein|search|ydaO]'' [Pubmed|22383849]
  • Regulatory mechanism

  • expression is controlled via termination antitermination by the [SW|ydaO riboswitch], expression is switched off upon binding od c-di-AMP [Pubmed|24141192,20511502]
  • Biological materials

    Mutant

  • 1A688 (''[protein|search|ydaO]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]
  • GP93 (''[protein|search|ydaO]''::''cat''), available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • lacZ fusion

  • GP2181 (based on [SW|pAC6]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP2405 ''ydaO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 25869574
  • Original publications

  • 15096624,20511502,23086297,24141192,25086509,25086507