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ydaO [2017-03-31 14:40:39]
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ydaO [2017-03-31 14:40:39]

putative transporter
locus
BSU04320
pI
9.93
mw
66.60 kDa
protein length
607 aa Sequence Blast
gene length
1821 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.3|Potassium uptake/ export]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|YdaO riboswitch]

    • Gene

    Coordinates
    486,432 -> 488,255

    The protein

    [SW|Localization]

  • cell membrane (according to [http://www.uniprot.org/uniprot/P96589 UniProt])

    • Expression and Regulation

    Operons

    genes
    [gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [regulon|ydaO riboswitch|ydaO riboswitch]: termination/antitermination, expression is switched off upon binding of c-di-AMP (formed at high potassium concentration) [pubmed|28420751], in [regulon|ydaO riboswitch|ydaO riboswitch]

    • regulation

  • expressed at low potassium concentration ([regulon|ydaO riboswitch|ydaO riboswitch]) [Pubmed|28420751]
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    Biological materials

    Mutant

  • MGNA-C090 (ydaO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2088 NBRP B. subtilis, Japan]
  • 1A688 (''[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A688&Search=1A688 BGSC]
  • GP93 (''[gene|5968E6D4D7378C69EC3E1E05B285069123AA1FD3|kimA]''::''cat''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP2913 (N-terminal His-tag, expression in ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • BP144 (based on [SW|pAC5]), available in [SW|Fabian Commichau]' and [SW|Jörg Stülke]'s labs

    • FLAG-tag construct

  • GP2405 ''ydaO-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 25869574

    • Original publications

  • 15096624,20511502,23086297,24141192,25086509,25086507