SubtiBank SubtiBank
rnpA [Thu Nov 24 2016 18:09:00 GMT+0100 (CET)]
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rnpA [Thu Nov 24 2016 18:09:00 GMT+0100 (CET)]

protein component of RNase P, metal-dependent 5 end maturation of precursor tRNAs
locus
BSU41050
pI
10.00
mw
13.00 kDa
function
cleavage of precursor sequences
from the 5 ends of pre-tRNAs
product
protein component of RNase P (substrate specificity)
essential
yes
ec
3.1.26.5
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|RNAses], [SW|translation], [SW|essential genes]
  • Gene

    Coordinates on the chromosome (coding sequence)
    4,214,753 -> 4,215,103

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from [protein|search|tRNA] precursor (according to Swiss-Prot)
  • 5' end maturation of tmRNA [Pubmed|25402410]
  • Protein family

  • rnpA family (according to Swiss-Prot)
  • [SW|Domains]

  • RNR motif: residues 60-68, stabilizes complexes with both the RNA part of [SW|RNase] P (''[protein|search|rnpB]'') and with pre-[protein|search|tRNA] ([protein|search|rnpB]-[protein|search|RnpA]-pre-[protein|search|tRNA]) [Pubmed|21622899]
  • Structure

  • [PDB|1A6F] [Pubmed|9563955]
  • [PDB|3Q1R] (complex of RnpA with the RNA [protein|search|RnpB] and [protein|search|tRNA], from ''Thermotoga maritima'') [Pubmed|21076397]
  • [SW|Interactions]

  • forms dimers [Pubmed|21764917]
  • [protein|search|RnpA]-[protein|search|RpmH]
  • [protein|search|RnpA]-[protein|search|RnpB] [Pubmed|21622899]
  • [protein|search|RnpA]-[protein|search|CshA] [Pubmed|21764917]
  • Expression and Regulation

    Regulation

  • expression transiently increases in the forespore [Pubmed|22848659]
  • Labs working on this gene/protein

  • [SW|Carol Fierke], University of Michigan, Ann Arbor, USA [https://www.chem.lsa.umich.edu/chem/faculty/facultyDetail.php?Uniqname=fierke homepage]
  • [SW|Roland Hartmann], Marburg University, Germany [http://www.pharmazie.uni-marburg.de/pharmchem/akhartmann/ag_hartmann.htm homepage]
  • References

    Reviews

  • 19215776,8901495,17868095,14691942,16650980,16980936,16679018,14550630
  • Original Publications

  • 10390342,17919279,17355991,11812129,16980484,11454206,19549719,11454206,9563955,19932118,17299131,20476778,11258888,16185070,21764917,22848659,21076397,20843005,21622899,24364358,25249623,25402410,26267651