ylaK

similar to phosphate starvation inducible protein [protein|6BF4F99DEF2E032A8547B920086305775D04FACF|PhoH]

locus: BSU_14810

pI: 5.42

mw: 49.17 kDa

protein length: 442 aa

gene length: 1329 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,549,465 1,550,793

The protein

Protein family

• C-terminal part: phoH family (with [protein|6BF4F99DEF2E032A8547B920086305775D04FACF|PhoH], according to UniProt)

Paralogous protein(s)

• [protein|6BF4F99DEF2E032A8547B920086305775D04FACF|PhoH]:

[SW|Domains]

• PINc domain (aa 3-135) (according to UniProt)

Structure

• [PDB|3B85] (aa 233-399, from Corynebacterium glutamicum, 38% identity)

Expression and Regulation

Operons

• genes: [gene|5913ABC121A03644897BD365D7E507661CD3AF39|ylaK]-[gene|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]
  description: [pubmed|22383849]
  regulation: expressed during [SW|sporulation] [pubmed|22383849]

Biological materials

Mutant

• MGNA-A540 (ylaK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/540 NBRP B. subtilis, Japan]
• BKE14810 ([gene|5913ABC121A03644897BD365D7E507661CD3AF39|ylaK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTCTCAGCCTCCTGATA, downstream forward: _UP4_CTTGCAGCTGATTTGCTGTA
• BKK14810 ([gene|5913ABC121A03644897BD365D7E507661CD3AF39|ylaK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTCTCAGCCTCCTGATA, downstream forward: _UP4_CTTGCAGCTGATTTGCTGTA

References

• 12762842,12107147