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citZ [Sun Nov 13 2016 11:06:21 GMT+0100 (CET)]
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citZ [Sun Nov 13 2016 11:06:21 GMT+0100 (CET)]

citrate synthase
locus
BSU29140
pI
5.00
mw
41.00 kDa
function
TCA cycle
product
citrate synthase II
essential
no
ec
2.3.3.1
synonyms
citA2
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|CcpC regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,981,151 -> 2,982,269

    Phenotypes of a mutant

  • glutamate auxotrophy and a defect in sporulation [Pubmed|8045898]

    • The protein

    Catalyzed reaction/ biological activity

  • Acetyl-CoA H2O oxaloacetate = citrate CoA (according to Swiss-Prot) [Pubmed| 8045898]

    • Protein family

  • citrate synthase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|MmgD], [protein|search|CitA]

    • Modification

  • phosphorylation on Ser-284 [Pubmed|17218307]

    • Effectors of protein activity

  • Inhibited by acetyl-CoA, 2-oxoglutarate and NADH [Pubmed|4211224] [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8RWC-SH&_user=5731894&_coverDate=01/01/1985&_rdoc=28&_fmt=high&_orig=browse&_srch=doc-info(#toc#4938#1985#998209998#270526#FLP#display#Volume)&_cdi=4938&_sort=d&_docanchor=&_ct=40&_acct=C000043105&_version=1&_urlVersion=0&_userid=5731894&md5=f14f4734123ab1177d7217cab6c7ce7d FEBS Letters]
  • Inhibited by citrate and CoA (competitively against acetyl-CoA and non-competitively against oxaloacetate) [Pubmed|4211224]
  • Inhibited by ATP competitively in ''B. subtilis'' strain 168 and HS 1A17 [Pubmed|4980242] [Pubmed|4211224]
  • In ''B. subtilis'' strain HS 2A2, ATP inhibits a non-competitive fashion [Pubmed|4211224]
  • Activated by AMP [Pubmed|4211224]

    • Structure

  • [PDB|2C6X]
  • [PDB|A discussion of citrate synthase structure]

    • [SW|Localization]

  • cytoplasm (homogeneously distributed throughout the cell) [Pubmed|24825009]

    • [SW|Interactions]

  • [protein|search|CitZ]-[protein|search|Mdh]-[protein|search|Icd] (this is the core of the TCA cycle metabolon) [Pubmed|20933603]

    • Additional information

  • extensive information on the structure and enzymatic properties of CitZ can be found at [http://www.proteopedia.org/wiki/index.php/Citrate_synthase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]'' [Pubmed|8045899]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8045899]

    • Regulation

  • repression by glucose (6.7-fold) ([protein|search|CcpA]) [Pubmed|12100558,12850135]
  • repression by glucose glutamate ([protein|search|CcpC]) [Pubmed|12100558]
  • reduced expression at excess citrate concentrations or iron depletion ([protein|search|CitB]) [Pubmed|23354745]

    • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression, [protein|search|CcpC]: transcription repression [Pubmed|12100558]
  • [protein|search|CcpC]: transcription repression (molecular inducer: citrate) [Pubmed|10656796]
  • [protein|search|CitB]: mRNA destabilization upon citrate accumulation or iron limitation [Pubmed|23354745]

    • Biological materials

    Mutant

  • GP678 (erm), GP797 (spec) available in [SW|Jörg Stülke]'s lab
  • 1A999 (''citZ''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A999&Search=1A999 BGSC]
  • GP790 (''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]''::''kan''), available in [SW|Jörg Stülke]'s lab
  • GP797 (''citZ''::''spec''), allows expression of ''[protein|search|icd]'' and ''[protein|search|mdh]'', available in [SW|Jörg Stülke]'s lab
  • GP1281 (''citZ''::''erm''), allows expression of ''[protein|search|icd]'' and ''[protein|search|mdh]'', available in [SW|Jörg Stülke]'s lab
  • GP2331 (''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]''::''kan''), Cre-recombinase is integrated in ''[protein|search|sacA]'',available in [SW|Jörg Stülke]'s lab
  • GP2333 (''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]''::''lox72''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP1120 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab)
  • pGP1776 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)
  • pGP1761 (expression with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP2515 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • available in [SW|Linc Sonenshein]'s lab

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • [SW|Jörg Stülke], University of Göttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

    • References

    Reviews

  • 3013232

    • Original publications

  • 10348849,8045899,22900538,10656796,12850135,17218307,12100558,9642180,8045898,8655569,4211224,4980242,20525796,24825009,20933603,23354745,24571712