ispC

1-deoxy-D-xylulose-5-phosphate reductoisomerase, second step in the MEP pathway of isoprenoid biosynthesis

locus: BSU_16550

pI: 5.80

mw: 43.12 kDa

protein length: 383 aa

gene length: 1152 bp

function: MEP pathway of isoprenoid biosynthesis

product: 1-deoxy-D-xylulose-5-phosphate reductoisomerase

essential: yes

ec: 1.1.1.267

synonyms: yluB,dxr

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 1,722,871 1,724,022

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• 2-C-methyl-D-erythritol 4-phosphate + NADP⁺ --> 1-deoxy-D-xylulose 5-phosphate + H⁺ + NADPH (according to UniProt)

Protein family

• DXR family (single member, according to UniProt)

Structure

• [PDB|1R0K] (from ''Zymomonas mobilis'', 45% identity) [Pubmed|15063313]

Expression and Regulation

Operons

• genes: [gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
  description: [Pubmed|22383849]

• genes: [gene|4A637EB9B400D30E5C6246D1E0EB5E6A30F38A16|uppS]-[gene|7E7F6BBA03C443A5AFA05A6D9352FEF1212A5AF6|cdsA]-[gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
  description: [Pubmed|22383849]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

References

• 17458547,15063313,21296950,23840410,26883633