SubtiBank SubtiBank
ispC
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

ispC

1-deoxy-D-xylulose-5-phosphate reductoisomerase, second step in the MEP pathway of isoprenoid biosynthesis
locus
BSU16550
pI
5.80
mw
43.12 kDa
protein length
388 aa Sequence Blast
gene length
1164 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
1-deoxy-D-xylulose-5-phosphate reductoisomerase
essential
yes
ec
1.1.1.267
synonyms
yluB,dxr

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,722,871 → 1,724,022

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH (according to Swiss-Prot)
  • Protein family

  • DXR family (according to Swiss-Prot)
  • Structure

  • [PDB|1R0K] (from ''Zymomonas mobilis'', 45% identity) [Pubmed|15063313]
  • Expression and Regulation

    Operons

    genes
    [gene|58CDF08A8F05C0BC25EB6E400316F85FD0F307E5|ispC]-[gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP]-[gene|848570563D3C92A8E1E6AB4DA530388D8AE94E75|proS]-[gene|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC]
    description
    [Pubmed|22383849]
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    additional information

  • the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
  • References

  • 17458547,15063313,21296950,23840410,26883633