SubtiBank SubtiBank
spoIIIAD
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

spoIIIAD

component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation
Locus
BSU_24400
Isoelectric point
4.73
Molecular weight
14.27 kDa
Protein length
133 aa Sequence Blast
Gene length
402 bp Sequence Blast
Function
activation of SigG
Product
component of a type III secretion system
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein secretion
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    2,535,544 2,535,945

    The protein

    Localization

  • membrane protein PubMed

    • Expression and Regulation

    Operons

    Genes
    yqhV-spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH
    Description
    PubMed

    Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon

    • Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

    • Additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed
    • view in new tab

    Genes
    spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH
    Description
    PubMed

    Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

    • Additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed
    • view in new tab

    Biological materials

    Mutant

  • BKE24400 (spoIIIAD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACAATGTCAATCTGCAAGC, downstream forward: _UP4_TCATAACCGAAAGGAGGCGG
  • BKK24400 (spoIIIAD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AACAATGTCAATCTGCAAGC, downstream forward: _UP4_TCATAACCGAAAGGAGGCGG

    • References

    Reviews

    Loading

    Original Publications

    Loading