Save the date! 20th International Conference on Bacilli and Gram-Positive Bacteria. Washington, D.C. July 23-26, 2019.

yvsH

putative lysine transporter

locus: BSU_33330

pI: 9.74

mw: 50.09 kDa

protein length: 469 aa

gene length: 1410 bp

function: uptake of lysine

product: putative lysine transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,419,656 3,421,065

The protein

Protein family

• [SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

Structure

• [PDB|3LRB] (from ''E. coli'', 32% identity) [Pubmed|19478139]

[SW|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|5762418690734FB959506B7317208CCA1A340720|yvsH]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|L-box|L-box]: transcription termination/antitermination, via a riboswitch, in [regulon|L-box|L-box]

Biological materials

Mutant

• MGNA-B067 (yvsH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1066 NBRP B. subtilis, Japan]
• BKE33330 ([gene|5762418690734FB959506B7317208CCA1A340720|yvsH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAGTACCTCCATGAT, downstream forward: _UP4_TAATGAAAAGACACCTGATT
• BKK33330 ([gene|5762418690734FB959506B7317208CCA1A340720|yvsH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCAGTACCTCCATGAT, downstream forward: _UP4_TAATGAAAAGACACCTGATT

References

• 18763711,14627808,19478139