feuB

[SW|ABC transporter ]for the siderophores Fe-enterobactin and Fe-bacillibactin (integral membrane protein)

locus: BSU_01620

pI: 9.54

mw: 35.74 kDa

protein length: 334 aa

gene length: 1005 bp

function: [SW|acquisition of iron]

product: [SW|ABC transporter ]for the siderophores Fe-enterobactin and Fe-bacillibactin (integral membrane protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 181,347 182,351

The protein

Protein family

• [SW|Binding-protein-dependent transport system permease family] (according to UniProt)
• [SW|FecCD subfamily] (according to UniProt)

Paralogous protein(s)

• [protein|BDAA56484E05DA3C1CB0CA09873A365657999679|FecD], [protein|3E1C925C31C00803982C8EB1C6B025C22DB4A828|FhuB]

Structure

• [PDB|4G1U] (heme transporter from Yersinia pestis, 33% identity) [pubmed|23142986]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]-[gene|57402D137E3D3791CAF0696421224F4E1DC1BA48|feuB]-[gene|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|feuC]-[gene|745167427618C4DA71848F9362043B66BC4E392E|ybbA]
  description: [Pubmed|12354229]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17725565], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]
  • [protein|EB28A65CECE994DF2DF486DEACF40F2533703DB0|Btr]: activation, in the presence of the co-activators bacillibactin or enterobactin [Pubmed|17725565], in [regulon|EB28A65CECE994DF2DF486DEACF40F2533703DB0|Btr regulon]

  regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
  • induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [pubmed|29133393]
  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

  additional information

  • the presence of an iron-responsive element bound by [SW|CitB] between ''[SW|feuA]'' and ''[SW|feuB]'' suggests iron-dependent regulation by [SW|CitB] [Pubmed|10468622]

Other regulations

• [protein|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|CitB]: translation control,

Biological materials

Mutant

• BKE01620 ([gene|57402D137E3D3791CAF0696421224F4E1DC1BA48|feuB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATACATACAAAAACCTACTC, downstream forward: _UP4_ATTAAACGAAAAGGAGGGGA
• BKK01620 ([gene|57402D137E3D3791CAF0696421224F4E1DC1BA48|feuB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATACATACAAAAACCTACTC, downstream forward: _UP4_ATTAAACGAAAAGGAGGGGA

References

• 19746494,16672620,10092453,16889643,17725565,12354229,10468622,29133393,23142986