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nprB [2018-02-13T06:03:56.000Z]
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nprB [2018-02-13T06:03:56.000Z]

extracellular neutral protease B
locus
BSU11100
pI
5.32
mw
59.17 kDa
protein length
538 aa Sequence Blast
gene length
1614 bp Sequence Blast
function
degradation of proteins
product
extracellular neutral protease B
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    1,186,037 → 1,187,653

    The protein

    Catalyzed reaction/ biological activity

  • Similar, but not identical, to that of thermolysin (according to Swiss-Prot)

    • Protein family

  • peptidase M4 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|309EAC680ABFF0B736E01F0B9BDFBB2FCBDB1BBF|NprE]

    • Structure

  • [PDB|5A3Y] (thermolysin, 44% identity) [pubmed|26527148]

    • [SW|Localization]

  • secreted (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|57139471EB143F6DCCF092DAD0FBB43B0D50D948|nprB]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [pubmed|31622331], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]

    • regulation

  • induced in biofilms ([SW|DegU]) [Pubmed|31622331]
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    Biological materials

    Mutant

  • KO7 (''ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr''), available as BGSC 1A1133
  • BKE11100 (Δ[gene|57139471EB143F6DCCF092DAD0FBB43B0D50D948|nprB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACACCACATCCTTCCT, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
  • BKK11100 (Δ[gene|57139471EB143F6DCCF092DAD0FBB43B0D50D948|nprB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAACACCACATCCTTCCT, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA

    • References

    Reviews

  • 20735481

    • Original publications

  • 24115457,26527148