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ndh [2018-06-29 17:26:13]
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ndh [2018-06-29 17:26:13]

NADH dehydrogenase (Menaquinone 7 & no proton)
locus
BSU12290
pI
6.29
mw
41.79 kDa
protein length
392 aa Sequence Blast
gene length
1176 bp Sequence Blast
function
respiration
product
NADH dehydrogenase (Menaquinone 7 & no proton)
essential
no
synonyms
yjlD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.4|Respiration/ other]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,299,074 → 1,300,252

    Phenotypes of a mutant

  • inactivation of ''ndh'' facilitates without a wall (due to reduction of oxidative stress) [Pubmed|26051891]

    • The protein

    Protein family

  • NADH dehydrogenase family (according to Swiss-Prot)

    • Structure

  • [PDB|4NWZ] (from ''C. thermarum'', 42% identity) [Pubmed|24444429]

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|8279630BE1D1D214162BA8E06FC05146F9ED517D|yjlC]-[gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]
    description
    [Pubmed|17015645]

    regulatory mechanism

  • [protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex]: repression, [Pubmed|17015645], in [regulon|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex regulon]
  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: repression, [pubmed|28439033], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]

    • regulation

  • induced at high NADH+ levels ([protein|B5EF521437323EF43F08E5EFDB5C798616CA499A|Rex]) [Pubmed|17015645]
  • repressed under anaerobic conditions ([protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]) [pubmed|28439033,10913079]
    • view in new tab

    genes
    [gene|2F5683B654BD1B7FA4D55011CCE6413C38DB3F4D|yjlB]-[gene|8279630BE1D1D214162BA8E06FC05146F9ED517D|yjlC]-[gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A358 (yjlD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/358 NBRP B. subtilis, Japan]
  • BKE12290 (Δ[gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTATATCCTCCGTCCT, downstream forward: _UP4_TAATCCTTTTAATGAATCTG
  • BKK12290 (Δ[gene|56F407D408272F3674612C9CDFE4A922680EC694|ndh]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTATATCCTCCGTCCT, downstream forward: _UP4_TAATCCTTTTAATGAATCTG

    • References

  • 17015645,20933603,11948165,18763711,15378759,26051891,24444429,28439033,10913079