SubtiBank SubtiBank
addB [Mon Aug 31 2015 13:53:31 GMT+0200 (CEST)]
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The 21st International Conference on Bacilli has been postponed to 2022 and will take place in Prague.

addB [Mon Aug 31 2015 13:53:31 GMT+0200 (CEST)]

ATP-dependent deoxyribonuclease (subunit B), required for efficient survival and replication restart after replication-transcription conflicts
locus
BSU10620
pI
5.39
mw
134.00 kDa
function
DNA repair/ recombination
product
ATP-dependent deoxyribonuclease (subunit B))
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [SW|Categories] containing this gene/protein

  • [SW|DNA repair/ recombination], [SW|genetic competence]
  • This gene is a member of the following [SW|regulons]

  • [SW|ComK regulon]
  • Gene

    Phenotypes of a mutant

  • ''[protein|search|recO] [protein|search|addA]-[protein|search|addB]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
  • The protein

    Catalyzed reaction/ biological activity

  • the enzyme is functional as a heterodimer of the [protein|search|AddA] and [protein|search|AddB] subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'→5' polarity located in the [protein|search|AddA] subunit [Pubmed|21071401]
  • the [protein|search|AddB] subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences [Pubmed|21071401]
  • Protein family

  • uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Structure

  • [PDB|3U4Q] (the [protein|search|AddA]-[protein|search|AddB]-DNA complex) [Pubmed|22307084]
  • [SW|Interactions]

  • [protein|search|AddA]-[protein|search|AddB] [Pubmed|10756102,21071401]
  • Expression and Regulation

    Operon

  • ''[protein|search|addB]-[protein|search|addA]-[protein|search|sbcD]-[protein|search|sbcC]-[protein|search|hlpB]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|7746142]
  • Regulation

  • positive control by [protein|search|ComK] [Pubmed|7746142]
  • Biological materials

    Mutant

  • GP1106 ([protein|search|addA]-[protein|search|addB], spc), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Mark Dillingham], Bristol, U.K. ([http://www.bris.ac.uk/biochemistry/research/md.html homepage])
  • References

    Reviews

  • 23202527,20116346,22933559,19542287,25486468
  • Original publications

  • 21809208,8387145,15610857,7746142,19129187,1646786,10756102,9781875,17570399,20350930,22307084,22383849,21071401,23056615,24682829,24670664,8752329,25939832,26001966,8510642