glxA

glyoxalase I

Locus: BSU_38370

Isoelectric point: 4.43

Molecular weight: 14.29 kDa

Protein length: 126 aa

Gene length: 381 bp

Function: detoxification of methylglyoxal

Product: glyoxalase I

Essential: no

Synonyms: ipa-18r,ywbC

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

Gene

Coordinates: 3,937,135 3,937,515

Phenotypes of a mutant

• increased sensitivity to methylglyoxal PubMed

The protein

Catalyzed reaction/ biological activity

• methylglyoxal + BSH - S-lactoyl-bacillithiol (BSH) PubMed

Protein family

• glyoxalase I family (with YraH and GlxB, according to UniProt)

Domains

• VOC domain (aa 4-126) (according to UniProt)

Structure

• 1F9Z (from E. coli, 35% identity) PubMed

Expression and Regulation

Operons

• Genes: glxA
  Description: PubMed

Biological materials

Mutant

• MGNA-B221 (ywbC::erm), available at the NBRP B. subtilis, Japan
• BKE38370 (glxA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTGCTCCTCCTCGTAA, downstream forward: _UP4_TAAAAATAAAGAACGTACAT
• BKK38370 (glxA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTGCTCCTCCTCGTAA, downstream forward: _UP4_TAAAAATAAAGAACGTACAT

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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