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purH
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purH

phosphoribosylaminoimidazole carboxamide formyltransferase
Locus
BSU_06520
Isoelectric point
5.13
Molecular weight
55.57 kDa
Protein length
512 aa Sequence Blast
Gene length
1539 bp Sequence Blast
Function
purine biosynthesis
Product
phosphoribosylaminoimidazole carboxamide formyltransferaseand inosine-monophosphate cyclohydrolase
Essential
no
E.C.
2.1.2.3
Synonyms
purJ

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
708,594 710,132

Phenotypes of a mutant

  • more resistant than wild-type to zinc limitation when grown on rich medium (LB), this is blocked by simultaneous deletion of purB (reason: accumulation of ZTP that stimulates the binding of ZagA to FolE, and thus folate biosynthesis during zinc limitation) PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • (6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide --> (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)
  • H2O + IMP --> 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)
  • Protein family

  • purH family (single member, according to UniProt)
  • Domains

  • MGS-like domain (aa 1-146) (according to UniProt)
  • Structure

  • 1ZCZ (from Thermotoga maritima, 36% identity, 54% similarity) PubMed
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • G-box: termination, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon
  • Regulation

  • expression activated by glucose (4.4 fold) PubMed
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    Biological materials

    Mutant

  • BKE06520 (purH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA
  • BKK06520 (purH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA
  • References

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