SubtiBank SubtiBank
purH
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purH

phosphoribosylaminoimidazole carboxamide formyltransferase
locus
BSU06520
pI
5.13
mw
55.57 kDa
protein length
512 aa Sequence Blast
gene length
1539 bp Sequence Blast
function
purine biosynthesis
product
phosphoribosylaminoimidazole carboxamide formyltransferaseand inosine-monophosphate cyclohydrolase
essential
no
ec
2.1.2.3
synonyms
purJ

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]
  • Gene

    Coordinates
    708,594 710,132

    The protein

    Catalyzed reaction/ biological activity

  • 10-formyltetrahydrofolate 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to Swiss-Prot)
  • Protein family

  • purH family (according to Swiss-Prot)
  • Structure

  • [PDB|1ZCZ] (from ''Thermotoga maritima'', 36% identity, 54% similarity) [Pubmed|18260100]
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|1A9F7847AA1EB2A264842B44CD1B1A21F0AF6E57|purE]-[gene|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|purK]-[gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB]-[gene|447D12E940C7F45B6C5015844F9DED9D513CD6E5|purC]-[gene|CCD4DA969086181CFA33D6D241F2CAAE02E0C758|purS]-[gene|DEE856E83E790EB57F103F7A9156408AF090995D|purQ]-[gene|D34203FAA84C19B34B3CB5F00AC2E2663FFA344D|purL]-[gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]-[gene|AD0DE9457D70DF02FA0F58867B24ABF236FAF65A|purM]-[gene|7B30F27D6C459437967A64467E52961F4A9E0F2C|purN]-[gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]-[gene|9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E|purD]
    description
    [Pubmed|3036807]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3036807], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|G-box|G-box]: termination, ([SW|riboswitch]) [pubmed|3036807,12787499], in [regulon|G-box|G-box]
  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR]: repression, (molecular inducer: PRPP) [Pubmed|7638212,2536750], in [regulon|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|PurR regulon]
  • regulation

  • expression activated by glucose (4.4 fold) [Pubmed|12850135]
  • view in new tab

    Biological materials

    Mutant

  • BKE06520 ([gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA
  • BKK06520 ([gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA
  • References

  • 3036807,12923093,15378759,7638212