You are currently viewing an outdated version of
SubtiWiki.
Please use
the newest version!
purH
phosphoribosylaminoimidazole carboxamide formyltransferase
Molecular weight
55.57 kDa
Function
purine biosynthesis
Product
phosphoribosylaminoimidazole carboxamide formyltransferaseand inosine-monophosphate cyclohydrolase
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
708,594 710,132
Phenotypes of a mutant
more resistant than wild-type to zinc limitation when grown on rich medium (LB), this is blocked by simultaneous deletion of purB (reason: accumulation of ZTP that stimulates the binding of ZagA to FolE, and thus folate biosynthesis during zinc limitation) PubMed The protein
Catalyzed reaction/ biological activity
(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide --> (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)H2O + IMP --> 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)Protein family
purH family (single member, according to UniProt)Domains
MGS-like domain (aa 1-146) (according to UniProt)Structure
1ZCZ (from Thermotoga maritima, 36% identity, 54% similarity) PubMed Additional information
Expression and Regulation
Operons
Sigma factors
Regulatory mechanism
Regulation
expression activated by glucose (4.4 fold) PubMed view in new tabBiological materials
Mutant
BKE06520 (purH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAABKK06520 (purH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA References
Loading