rbgA [Tue Dec 13 2016 11:16:33 GMT+0100 (CET)]

assembly of the 50S subunit of the ribosome

locus: BSU16050

pI: 9.00

mw: 31.00 kDa

function: ribosome assembly

product: GTPase

essential: yes

synonyms: ylqF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.3|Targets of (p)ppGpp]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|essential genes], [SW|GTP-binding proteins]

Gene

Coordinates on the chromosome (coding sequence): 1,676,532 -> 1,677,380

Phenotypes of a mutant

• essential [Pubmed|12682299]
• depletion confers a slow growth phenotype, and induces an abnormality of the ''in vivo'' ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes [Pubmed|24335279,23700310]
• premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including [rplP|search|L16], [rpmA|search|L27], [rpmB|search|L28], [rpmGA|search|L33], [rpmI|search|L35] and [rpmJ|search|L36] [Pubmed|24335279,23700310]

The protein

Catalyzed reaction/ biological activity

• proper positioning of [rplF|search|L6] in the large subunit of the [SW|ribosome] prior to incorporation of [rplP|search|L16] [Pubmed|25330043]
• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• MTG1 subfamily (according to Swiss-Prot) Era/Obg family

Paralogous protein(s)

• [protein|search|Era], [protein|search|Obg], [protein|search|YphC], [protein|search|YsxC], [protein|search|RbgA], [protein|search|YqeH], [protein|search|ThdF], [protein|search|YyaF], [protein|search|YnbA]

[SW|Domains]

• RNA-binding domain at the C-terminus [Pubmed|23325847]

Effectors of protein activity

• GTPase activity requires binding of [rplP|search|L16] to the 50S subunit of the [SW|ribosome] [Pubmed|24335279]
• binds ppGpp [Pubmed|26951678], ppGpp may inhibit activity [Pubmed|26951678]

Structure

• [PDB|1PUJ]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

[SW|Interactions]

• [protein|search|RbgA]-[protein|search|RplF] [Pubmed|25330043]

Labs working on this gene/protein

• [SW|Naotake Ogasawara], Nara, Japan

References

Reviews

• 19575570,21885683

Original publications

• 12427945,16390447,16997968,23325847,22267738,23700310,24335279,25330043,26951678