yacD

putative peptidyl-prolyl cis-trans isomerase, similar to secretion protein [protein|6EE13E61A218A6D52BAC83A1145B0B96961CE289|PrsA]

locus: BSU_00720

pI: 4.95

mw: 33.94 kDa

protein length: 297 aa

gene length: 894 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.6|Protein secretion/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 80,802 81,695

The protein

Catalyzed reaction/ biological activity

• [protein]-peptidylproline (ω=180) --> [protein]-peptidylproline (ω=0) (according to UniProt)
• peptidyl-prolyl cis-trans isomerase activity (according to UniProt)

[SW|Domains]

• PpiC domain (aa 154-247) (according to UniProt)

Structure

• [PDB|5EZ1] (from Helicobacter pylori, 26% identity) [pubmed|26850168]

[SW|Localization]

• extracellular (with signal peptide) (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|9B8EAD4EDDA97526D4462F6F3205F81FECD039DE|coaX]-[gene|D579A7E4D8C3DEE4F85586C5F721CAD5FE2BCA75|yacC]-[gene|548926987569033AA0C40610B8E80FB2BB8E1E3A|yacD]-[gene|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [pubmed|30480837], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • induced by heat stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|30480837]

Biological materials

Mutant

• MGNA-B925 (yacD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1924 NBRP B. subtilis, Japan]
• BKE00720 ([gene|548926987569033AA0C40610B8E80FB2BB8E1E3A|yacD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACCTGCTTTCAGCCCCT, downstream forward: _UP4_TGATTGACAAAAAATTTTGA
• BKK00720 ([gene|548926987569033AA0C40610B8E80FB2BB8E1E3A|yacD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACCTGCTTTCAGCCCCT, downstream forward: _UP4_TGATTGACAAAAAATTTTGA