gerPE

spore germination protein, facilitates access of nutrient germinants to their cognate germinant receptors in spores inner membrane

locus: BSU_10680

pI: 10.71

mw: 14.67 kDa

protein length: 133 aa

gene length: 402 bp

function: germination

product: unknown

essential: no

synonyms: yisD

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]

Gene

Coordinates: 1,148,744 1,149,145

Expression and Regulation

Operons

• genes: [gene|C4DA51243A4249B2489133C8EF0F8A30B05F4C86|gerPA]-[gene|B52A09BF195FE8E143FCB2EF5EBA53454A4ED3D5|gerPB]-[gene|2C49C261D2C7EEDD8E01A384917B63B6965B4AB9|gerPC]-[gene|F8658BB6D52793F8EC289FEC652241D729D72BF6|gerPD]-[gene|53833A2E7176C04AF2E3F7CD7FC9928DC1A0110D|gerPE]-[gene|C2DDBBD801BC61B89024198624360CC55D208247|gerPF]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: repression, in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,10715007]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|gerPA]' and '[protein|search|yisI]' [PubMed|20525796]

Biological materials

Mutant

• MGNA-B203 (yisD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1202 NBRP B. subtilis, Japan]
• BKE10680 ([gene|53833A2E7176C04AF2E3F7CD7FC9928DC1A0110D|gerPE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTCTGGCGCAAGCGG, downstream forward: _UP4_TAATAAACAGAGACTTCGAT
• BKK10680 ([gene|53833A2E7176C04AF2E3F7CD7FC9928DC1A0110D|gerPE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTCTGGCGCAAGCGG, downstream forward: _UP4_TAATAAACAGAGACTTCGAT

References

• 10715007