SubtiBank SubtiBank
metK
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

metK

S-adenosylmethionine synthetase
locus
BSU30550
pI
5.03
mw
43.88 kDa
protein length
400 aa Sequence Blast
gene length
1200 bp Sequence Blast
function
S-adenosylmethionine synthesis
product
S-adenosylmethionine synthetase
essential
yes
ec
2.5.1.6
synonyms
metE

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,127,825 → 3,129,027

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)
  • Protein family

  • AdoMet synthetase family (according to Swiss-Prot)
  • Structure

  • [PDB|1FUG] (from ''Escherichia coli'', 63% identity, 75% similarity) [Pubmed|8723769]
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|52D4DA0AA45F0774543786B55D18DF9DF53A5029|metK]
    description
    [Pubmed|9387221]

    regulatory mechanism

  • [regulon|S-box|S-box]: transcription termination, via [protein|C245476CE35028167510F67153F392D91447553F|MgtE riboswitch] binding S-adenosylmethionine, in [regulon|S-box|S-box]
  • regulation

  • induced by methionine starvation ([protein|search|S-box]) [Pubmed|10094622]
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    Biological materials

    lacZ fusion

  • pGP432 (in [protein|search|pAC7]), available in [SW|Stülke] lab
  • References

  • 10094622,16672621,9387221,18039762,15378759