yoaI

similar to oxygenase component of 4-hydroxyphenylacetate 3-monooxygenase

locus: BSU_18620

pI: 5.13

mw: 43.01 kDa

protein length: 483 aa

gene length: 1452 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,031,439 2,032,890

The protein

Catalyzed reaction/ biological activity

• 4-hydroxyphenylacetate + FADH₂ + O₂ --> 3,4-dihydroxyphenylacetate + FAD + H⁺ + H₂O (according to UniProt)

Protein family

• FADH(2)-utilizing monooxygenase family (single member, according to UniProt)

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|2YYG] (from ''Thermus thermophilus'', 42% identity) [Pubmed|17804419]

Expression and Regulation

Operons

• genes: [gene|5224B7AD3B669120DBCC071540F6B37D11FD5260|yoaI]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A838 (yoaI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/838 NBRP B. subtilis, Japan]
• BKE18620 ([gene|5224B7AD3B669120DBCC071540F6B37D11FD5260|yoaI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAGTTTCGCTCCTTC, downstream forward: _UP4_TGAAAAAACAGCGGGAAAAG
• BKK18620 ([gene|5224B7AD3B669120DBCC071540F6B37D11FD5260|yoaI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAGTTTCGCTCCTTC, downstream forward: _UP4_TGAAAAAACAGCGGGAAAAG

References

• 17804419