cysH

phosphoadenosine phosphosulfate sulfotransferase

locus: BSU_15570

pI: 5.46

mw: 26.83 kDa

protein length: 233 aa

gene length: 702 bp

function: sulfate reduction

product: phosphoadenosine phosphosulfate sulfotransferase

essential: no

ec: 1.8.4.8

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]

Gene

Coordinates: 1,630,382 1,631,083

The protein

Catalyzed reaction/ biological activity

• [thioredoxin]-disulfide + adenosine 3',5'-bisphosphate + 2 H⁺ + sulfite --> 3'-phosphoadenylyl sulfate + [thioredoxin]-dithiol (according to UniProt)

Protein family

• PAPS reductase family (with [protein|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|YitB], according to UniProt)

Paralogous protein(s)

• [protein|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|YitB]

Structure

• [PDB|2GOY] (from ''Pseudomonas aeruginosa'', 35% identity) [Pubmed|17010373]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE15570 ([gene|51EB09C122A7166D662AE72842A44EC5FBA03134|cysH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCTTTCCTCCTTT, downstream forward: _UP4_CTGCATGAATAAGGAGCTGC
• BKK15570 ([gene|51EB09C122A7166D662AE72842A44EC5FBA03134|cysH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15570 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTCTTTCCTCCTTT, downstream forward: _UP4_CTGCATGAATAAGGAGCTGC

labs

• [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

References

• 10094622,16267287,11004190,12107147,18039762,9006060,17010373,29794222