hemQ [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

heme-binding protein, essential for heme biosynthesis

locus: BSU37670

pI: 4.00

mw: 29.00 kDa

function: biosynthesis of heme

product: heme-binding protein

essential: no

ec: 1.11.1.-

synonyms: ipa-87r, ywfI

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.6|Biosynthesis of heme/ siroheme]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis of cofactors], [SW|phosphoproteins]

Gene

Coordinates on the chromosome (coding sequence): 3,866,596 -> 3,867,360

Phenotypes of a mutant

• defect in heme biosynthesis

The protein

Protein family

• UPF0447 family (according to Swiss-Prot)

Modification

• phosphorylation on Ser-41 [Pubmed|17218307]

[SW|Cofactors]

• heme

Structure

• [PDB|1T0T] (''Geobacillus stearothermophilus'')

Expression and Regulation

Operon

• ''hemQ'' [Pubmed|9353933]

Biological materials

Expression vector

• pGP2643: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
• pGP2644: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
• pGP2637: N-terminal Strep-tag, in [SW|pGP380], for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

• 17218307,9353933,20543190