hemQ

coproheme decarboxylase

locus: BSU_37670

pI: 4.97

mw: 29.35 kDa

protein length: 254 aa

gene length: 765 bp

function: biosynthesis of heme

product: coproheme decarboxylase

essential: no

ec: 1.11.1.-

synonyms: ipa-87r,ywfI

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.6|Biosynthesis of heme/ siroheme]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 3,866,596 3,867,360

Phenotypes of a mutant

• defect in heme biosynthesis

The protein

Catalyzed reaction/ biological activity

• coproheme --> protoheme + 2 CO₂ + 4 H⁺ [pubmed|25646457]

Protein family

• UPF0447 family (single member, according to UniProt)

Modification

• phosphorylation on Ser-41 [Pubmed|17218307]

[SW|Cofactors]

• heme

Structure

• [PDB|5T2K] (''Geobacillus stearothermophilus'') [pubmed|27936663]

Expression and Regulation

Operons

• genes: [gene|51B3ED39FC423164A6FD6271815E029137B362A9|hemQ]
  description: [Pubmed|9353933]

Biological materials

Mutant

• MGNA-A516 (ywfI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/516 NBRP B. subtilis, Japan]
• BKK37670 ([gene|51B3ED39FC423164A6FD6271815E029137B362A9|hemQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTATCTTCACTCCCATT, downstream forward: _UP4_TAATATCCCCTCCCGCCCTA

Expression vectors

• pGP2643: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
• pGP2644: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
• pGP2637: N-terminal Strep-tag, in [SW|pGP380], for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 28123057,25711532

Original Publications

• 17218307,9353933,20543190,25646457,27758026,27982566,27599156,27936663,26083961,27597779