yhfT

similar to long-chain fatty-acid-CoA ligase, involved in surfactin production

locus: BSU_10360

pI: 5.95

mw: 52.71 kDa

protein length: 479 aa

gene length: 1440 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,110,479 1,111,918

The protein

Protein family

• [SW|ATP-dependent AMP-binding enzyme family] (according to UniProt)

Paralogous protein(s)

• [protein|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|YngI], [protein|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|LcfA], [protein|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|LcfB]

Structure

• [PDB|5BUQ] ([protein|16412BAB04BBF9FD1C3653328923687874C81868|MenE], 29% identitiy) [Pubmed|26276389]

Expression and Regulation

Operons

• genes: [gene|22215066A7BEA4E53189DE6639C19D1AD86DA7F4|yhfU]-[gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]-[gene|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|yhfS]-[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA]: repression, [Pubmed|11717296], in [regulon|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA regulon]

  regulation

  • repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|11717296]

  additional information

  • weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]

• genes: [gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]-[gene|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|yhfS]-[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]
  description: [Pubmed|22383849]

  additional information

  • weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]

Biological materials

Mutant

• MGNA-B280 (yhfT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1279 NBRP B. subtilis, Japan]
• available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]
• BKE10360 ([gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTATGAGTAATTGTCATGT, downstream forward: _UP4_CTGGAAGAGAGTGTACAGTA
• BKK10360 ([gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTATGAGTAATTGTCATGT, downstream forward: _UP4_CTGGAAGAGAGTGTACAGTA

References

• 20797616,22383849,12368242