serB [2019-03-22 10:21:35]

phosphoserine phosphatase, catalyzes the last step in serine biosynthesis

locus: BSU28940

pI: 4.67

mw: 29.36 kDa

protein length: 260 aa

gene length: 783 bp

function: biosynthesis of serine

product: phosphoserine phoshatase

essential: no

synonyms: undefined,ysaA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.8|Biosynthesis/ acquisition of serine/ glycine/ alanine]

Gene

Coordinates: 2,958,434 2,959,216

Phenotypes of a mutant

• auxotrophic for serine [Pubmed|28189581]

The protein

Catalyzed reaction/ biological activity

• phosphoserine - serine + phosphate [Pubmed|28189581]

Protein family

• [SW|HAD superfamily]

Paralogous protein(s)

• [protein|2E1E22349750C3EE9B6575EED30A82ED0D5F07F0|YfnB]

Structure

• [PDB|2GFH] (from mouse, 32% identity)

Expression and Regulation

Operons

• genes: [gene|50417B2FA0ED2258A13B1E708514F055297599EF|serB]
  description: [pubmed|22383849,8969504]

Biological materials

Mutant

• MGNA-A998 [gene|50417B2FA0ED2258A13B1E708514F055297599EF|serB]::erm, available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/998 NBRP B. subtilis, Japan]
• BKE28940 ([gene|50417B2FA0ED2258A13B1E708514F055297599EF|serB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCTCCTCTGCTT, downstream forward: _UP4_TAAAAAAAGCATGATCTCTT
• BKK28940 ([gene|50417B2FA0ED2258A13B1E708514F055297599EF|serB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28940 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATCTTCTCCTCTGCTT, downstream forward: _UP4_TAAAAAAAGCATGATCTCTT

References

• 8969504,27784292,28189581