glnL

two-component response regulator, regulation of the glsA-glnT operon

locus: BSU_02450

pI: 7.02

mw: 35.56 kDa

protein length: 314 aa

gene length: 945 bp

function: regulation of the glsA-glnT operon

product: two-component response regulator

essential: no

synonyms: ycbB,yzgB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

Gene

Coordinates: 266,719 267,663

The protein

[SW|Domains]

• [SW|Response regulatory domain] (aa 2–118) (according to UniProt)

Modification

• phosphorylated by [protein|C18B886D06879C64B64606371E85A7D4D22DCFAC|GlnK] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|C18B886D06879C64B64606371E85A7D4D22DCFAC|glnK]-[gene|4E6EA2EABBDAA427E8F9EE1AE1800AF8657CF18B|glnL]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C026 (ycbB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2024 NBRP B. subtilis, Japan]
• BKE02450 ([gene|4E6EA2EABBDAA427E8F9EE1AE1800AF8657CF18B|glnL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCTTTTGGTTCACCCT, downstream forward: _UP4_TAAACCAAACAGCCGGCTGA
• BKK02450 ([gene|4E6EA2EABBDAA427E8F9EE1AE1800AF8657CF18B|glnL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCTTTTGGTTCACCCT, downstream forward: _UP4_TAAACCAAACAGCCGGCTGA

References

• 10094672,15995196