yckE

aryl--glucosidase

locus: BSU_03410

pI: 5.57

mw: 54.97 kDa

protein length: 477 aa

gene length: 1434 bp

function: utilization of aryl--glucosides

product: aryl--glucosidase

essential: no

ec: 3.2.1.86

synonyms: bglC

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.18|Utilization of beta-glucosides]

Gene

Coordinates: 370,259 371,692

The protein

Catalyzed reaction/ biological activity

• 6-phospho-β-D-glucosyl-(1→4)-D-glucose + H₂O --> D-glucose + D-glucose 6-phosphate (according to UniProt)

Protein family

• [SW|glycosyl hydrolase 1 family] (according to UniProt)

Structure

• [PDB|4ZE4] (from Geobacillus stearothermophilus, 72% identity)

Expression and Regulation

Operons

• genes: [gene|4E626CEED2AB27442B49C199CAFDA89333D44715|yckE]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C055 (yckE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2053 NBRP B. subtilis, Japan]
• BKE03410 ([gene|4E626CEED2AB27442B49C199CAFDA89333D44715|yckE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTGTTTATCACTCCTG, downstream forward: _UP4_TAAAGAGTCCCTGAGAGTTA
• BKK03410 ([gene|4E626CEED2AB27442B49C199CAFDA89333D44715|yckE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGTGTTTATCACTCCTG, downstream forward: _UP4_TAAAGAGTCCCTGAGAGTTA

References

• 14652714,15139916,18069788