SubtiBank SubtiBank
trxA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

trxA

thioredoxin, antioxidative action by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange, important for the prevention of protein aggregation during severe heat stress
locus
BSU28500
pI
4.31
mw
11.25 kDa
protein length
104 aa Sequence Blast
gene length
312 bp Sequence Blast
function
protection of proteins against oxidative damage
product
thioredoxin
essential
yes
synonyms
trx

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,913,024 → 2,913,338

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Protein family

  • thioredoxin family (according to Swiss-Prot)
  • Structure

  • [PDB|2GZZ] (oxidized form), [PDB|2GZY] (reduced form), [PDB|2IPA] (TrxA-ArsC-complex) [Pubmed|17303556]
  • [SW|Localization]

  • cytoplasm [Pubmed|17501922]
  • Expression and Regulation

    Operons

    genes
    [gene|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|trxA]
    description
    [Pubmed|9537387]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9537387], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|9537387,11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [Pubmed|9852015], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]
  • regulation

  • induced by heat stress ([protein|search|CtsR]) [Pubmed|9852015]
  • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|trxA]' and '[protein|search|abf2]' [PubMed|20525796]
  • view in new tab

    References

    Reviews

  • 23899494
  • Original publications

  • 9537387,15937154,18601268,18456801,9852015,11544224,18687074,20084284,17303556,12642660,20525796,17501922,24401092,24417481